Motivation is one of the most critical elements in the area of Human Resources Management. Enterprises design motivation systems not only to encourage employees to perform their best in the most efficient way, but also to attract future candidates to apply for specific posts. This is an in-depth study of some selected business professionals in Dhaka, Bangladesh and the purpose of our research is to find the main factors affecting motivation amongst the employees of some selected organizations. A questionnaire survey was undertaken among 45 employees of different professionals to analyze their perceptions regarding motivation in workplace. Required data are collected through questionnaires and the instrument for data collection is comprised of 5 points Likert Scale. Findings revealed that employee motivation is influenced by these main factors- Sufficient salary, promotion, recognition, being part of a team, open communication, good working environment, job security, freedom to plan and participation in goal setting. This study would be a prelude and of great help to managers and Human Resources professionals to raise the productivity of the company by increasing the motivation of their employees.